Chemiluminescence Determination of Cefixime Trihydrate Based on Acidic Diperiodatoargentate(Ⅲ)-Rhodamine 6-G System

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（5）
• 页码范围: 775-781
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s40242-019-9073-x

摘要

A novel chemilnminescence(CL) method is proposed for cefixime trihydrate(CFX) determination based on its enhancement effect on diperiodatoargentate(Ⅲ)(DPA)-rhodamine 6-G(Rh6-G) reaction in conjunction with flow injection analysis(FIA).A linear calibration curve was achieved over the range from 0.01 mg/L to 2.5 mg/L CFX(R2=0.999,n=8) with relative standard deviation(RSD) of 1.4％-3.8％(n=4)),limit of detection(LOD) of 3.0×10-3 mg/L(S/N=3),injection throughput of 180/h and regression equation ofy=1 113.2x-14.596[y=CL intensity (mV) and x=concentration of CFX(mg/L)].The method was successfully applied to CFX determination in pharmaceutical formulations and the recoveries(％) for proposed FI-CL and a reported spectrophotometric method by applying the Student t-test [calculated t-test value:t=1.079215,and tabulated t-distributed(95％)=2.200985] were not significantly different.The CFX was efficiently extracted and no significant effect of commonly found excipients in the pharmaceutical formulations was observed.The mechanism of CL reaction is discussed briefly.