Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review

• 作者: Christoph Schmitz Christopher Alt Eckhard U Alt Maren C Kiessling Onder Solakoglu Werner Gotz
• 来源期刊: 世界干细胞杂志:英文版(电子版)
• 学科: 医学
• 关键词: Case report Cell-based therapy Guided bone regeneration MAXILLARY SINUS augmentation Lateral ALVEOLAR ridge augmentation Unmodified AUTOLOGOUS adiposederived REGENERATIVE cells Stem
• 年，卷（期）: 2019,（2）
• 页码范围: 124-146
• 页数: 23页
• 分类号: R

摘要

BACKGROUND Novel strategies are needed for improving guided bone regeneration (GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation (GBR-MSA) and in lateral alveolar ridge augmentation (LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells (UA-ADRCs), fraction 2 of plasma rich in growth factors (PRGF-2) and an osteoinductive scaffold (OIS)(UAADRC/ PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone (PRGF-2/OIS) in GBR-MSA/LRA. CASE SUMMARY A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBRMSA/ LRA. At the latter time point implants were placed. Radiographs (32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic, histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS. CONCLUSION GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.