Patchouli alcohol induces autophagy in human lung adenocarcinoma cells A549 via increasing ratio of LC3 protein II/I and down-regulating p62

• 作者: Hao-Tian Wen Liu Yang Qi Li Qing Ji Ru Jia
• 来源期刊: 精准医学研究
• 学科: 医学
• 关键词: Patchouli alcohol AUTOPHAGY A549 cells LC3 PROTEIN Proliferation
• 年，卷（期）: 2019,（1）
• 页码范围: 26-31
• 页数: 6页
• 分类号: R

摘要

Objective:To study the patchouli alcohol(PA)-induced autophagy and proliferation inhibition of lung cancer A549 cells.Methods:the effects of PA at different concentrations on the proliferation and autophagy of A549 cells were detected by Cell Counting Kit-8(CCK-8)and electron microscopy,respectively.The A549 cells overexpressing LC3 gene were constructed by double luciferase lentiviral vector,and were treated with PA at half-inhibitory concentration(IC50)for 24 hours.Fluorescence microscopy was used to observe the fluorescence accumulation in cells.Western blot was used to detect the ratio of LC3 proteinⅡ/Ⅰ.Results:IC50 of PA on A549 cells for 24 h was 300μM.Treatment with 150μM and 300μM of PA for 24 h induced a cytoplasmic autophagic vesicle-like structure appearance in the cytoplasm of A549 cells,and the encapsulated cytoplasmic components were observed in those vesicles.150μM and 300μM of PA also elicited LC3 fluorescent protein accumulation(P<0.05).With the increased concentrations of PA,the ratios of LC3 proteinⅡ/Ⅰwere elevated(P<0.05),and the expressions of autophagy-related protein p62 were decreased gradually(P<0.05).Conclusion:PA can induce autophagy of lung cancer cells A549 by up-regulating the ratio of LC3 proteinⅡ/Ⅰand down-regulating p62 expression,which eventually inhibited the proliferation of lung cancer cells.