Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy

• 来源期刊: 生物物理学报
• 年，卷（期）: 2019,（4）
• 页码范围: 176-183
• 页数: 8页
• 语种: 中文

摘要

Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage.Here,we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization.Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance.The experimental result indicated that the DNA stretching success ratio was improved from 0.89％ to 13.5％.This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.