Preparation of Zn<sup>2+</sup>-Chelated Carboxymethyl Poly(1-Vinylimidazole) for Intracellular Zn<sup>2+</sup>Delivery

• 作者: Akito Endo Shoichiro Asayama
• 来源期刊: 矿物质和材料特性和工程(英文)
• 学科: 化学
• 关键词: Zinc Ion (Zn2+) CARBOXYMETHYL Poly(1-Vinylimidazole) INTRACELLULAR Delivery
• 年，卷（期）: 2019,（5）
• 页码范围: 213-220
• 页数: 8页
• 分类号: O6

摘要

Zinc ions (Zn2+), known to be a novel intracellular second messenger related to various biological functions, have been delivered inside cells. For the intracellular Zn2+ delivery, Zn2+ has been chelated to carboxymethyl poly(1-vinylimidazole) (CM-PVIm) by mixing zinc chloride (ZnCl2) or zinc acetate (Zn(OAc)2) with CM-PVIm. The resulting Zn2+-chelated CM-PVIm, that is, Zn2+/CM-PVIm complex by mixing ZnCl2 exhibited smaller particle size below 10 nm and possessed larger amount of Zn2+ ions, as compared to the Zn2+/CM-PVIm by mixing Zn(OAc)2. The both Zn2+/CM-PVIm complexes exhibited no significant cytotoxicity, leading to intracellular Zn2+ delivery. The Zn2+/CM-PVIm by mixing ZnCl2 delivered larger amount of intracellular Zn2+ ions than that by mixing Zn(OAc)2. These results suggest that the optimal Zn2+/CM-PVIm complex is a useful tool for intracellular Zn2+ delivery to control various biological functions.