Patrinia scabiosaefolia Inhibits Growth of 5-FU-Resistant Colorectal Carcinoma Cells via Induction of Apoptosis and Suppression of AKT Pathway

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2019,（2）
• 页码范围: 116-121
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11655-018-3002-6

摘要

Objective:To investigate the effects of ethanol extract of Patrinia scabiosaefolia (EEPS) on chemo-resistance of colorectal cancer cells (CRC) and explore the possible molecular mechanisms.Methods:5-fluorouracil (5-FU)-resistant human colorectal carcinoma cell line (HCT-8/5-FU) and its parental cells HCT-8 were treated with EEPS (0,0.25,0.50,1 or 2 mg/mL),or 5-FU (0,100,200,400,800 or 1600 μmol/L).The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the cell viability.Cell density was observed by phase-contrast microscope,cell counting and colony formation assay were used to determine the cell proliferation of HCT-8/5-FU cells treated with 0,0.5,1 or 2 mg/mL EEPS.Cell apoptosis was determined by Hoechst staining.Western-blot was performed to detect the phosphorylation of AKT as well as the protein expression level of B-cell CLL/lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax).Results:Compared with HCT-8 cells,MTT assay results indicated that HCT-8/5-FU cells were resistant to 5-FU treatment (P＜0.05),and sensitive to EEPS treatment (P＞0.05).Moreover,compared with untreated HCT-8/5-FU cells,1 and 2 mg/mL of EEPS treatment significantly reduced cell density,cell number,inhibited cell survival (P＜0.05),and induced apoptosis in HCT-8/5-FU cells.Furthermore,1 and 2 mg/mL of EEPS significantly decreased the phosphorylation level of p-AKr and Bcl-2 protein expression,and increased the expression of Bax protein (P＜0.05).Conclusion:EEPS is a promising therapeutic agent that may overcome chemo-resistance in cancer cells,likely through suppression of the AKT pathway and promotion of cancer cell apoptosis.