Two chemical-controlled switchable Cas9s for tunable gene editing

• 来源期刊: 生物物理学报
• 年，卷（期）: 2019,（3）
• 页码范围: 161-165
• 页数: 5页
• 语种: 中文

摘要

INTRODUCTION The clustered regularly interspaced short palindromic repeats [CRISPR] loci and their associated [Cas] genes are found in many bacterial and archaeal genomes as adaptive defense systems against phage infection and plasmid transfer [Mohanraju et al.2016].In the CRISPR-Cas system,single or multiple Cas proteins were complexed with a small CRISPR RNA [crRNA],forming an RNA protein complex [RNP],which uses the crRNA as the guide to destruct the invading nucleic acids [Mohanraju et al.2016].