Shexiang Tongxin Dropping Pill（麝香通心滴丸）Protects against Na2S2O4-lnduced Hypoxia-Reoxygenation Injury in H9c2 Cells

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2019,（6）
• 页码范围: 439-445
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11655-018-2976-4

摘要

Objectives:To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STP) on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells.Methods:The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining,annexin V/propidium iodide (PI) flow cytometry,real-time polymerase chain reaction and Western blot analysis.Results:STP pretreatment significantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment (P＜0.05).In addition,STP pretreatment attenuated Na2S2O4-induced hypoxic damage,down-regulated the expression of pro-apoptotic Bax,and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells (P＜0.05).Conclusions:STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis.These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.