The road lighting system and the road greening system, which are mutually interrelated and independent, are two important parts in the urban road environment. Unreasonable road lighting is easy to induce light pollution and has a great negative impact on the physiology and growth of garden plants in the urban green space. In this paper, 21 kinds of common tree species in the urban green space of Zhengzhou were selected as the research object, and the photosynthetic physiological parameters of landscape trees under the LED light source were observed using LI-6400 Photosynthesis System. This paper attempted to find the critical point for initiating photosynthesis of different types of tree species under a certain light source and then calculated the safe distance between lighting fixtures and landscape trees. The results showed that road lighting interfered with the photosynthetic physiological activities of the surveyed trees, affecting the normal dormancy of the plants at night;the sensitivity of different tree species to night lighting was different, and there were some differences in the light compensation points, so the corresponding safe distance was also different. It is hoped that this study can provide a valuable reference and scientific basis for urban road greening and lighting design.