Strawberry Notch 1 (SBN01) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice

• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2019,（4）
• 页码范围: 345-350
• 页数: 6页
• 语种: 英文
• DOI: 10.4103/aja.aja_65_18

摘要

While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells,the mechanisms of SSC self-renewal,proliferation,and differentiation remain poorly understood.We have previously identified Strawberry Notch 1 (SBN01),a vertebrate strawberry notch family protein,in the proteome profile for mouse SSC maturation and differentiation,revealing SBN01 is associated with neonatal testicular development.To explore further the location and function of SBN01 in the testes,we performed Sbno1 gene knockdown in mice to study the effects of SBN01 on neonatal testicular and SSC development.Our results revealed that SBN01 is required for neonatal testicular and SSC development in mice.Particularly,in vitro Sbno1 gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway,through Jun N-terminal kinases.Our study suggests SBN01 maintains SSCs by promoting the noncanonical Wnt pathway.