Up-regulation of P2X7 Receptors Contributes to Spinal Microglial Activation and the Development of Pain Induced by BmK-Ⅰ

• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2019,（4）
• 页码范围: 624-636
• 页数: 13页
• 语种: 英文

摘要

Previous work has demonstrated that the sensitization of spinal neurons and microglia is important in the development of pain behaviors induced by BmK Ⅰ,a Na+ channel activator and a major peptide component of the venom of the scorpion Buthus martensi Karsch (BmK).We found that the expression of P2X7 receptors (P2X7Rs) was up-regulated in the ipsilateral spinal dorsal horn after BmK Ⅰ injection in rats.P2X7R was selectively localized in microglia but not astrocytes or neurons.Similarly,interleukin 1β (IL-1β) was selectively up-regulated in microglia in the spinal dorsal horn after BmK Ⅰ injection.Intrathecal injection of P2X7R antagonists largely reduced BmK Ⅰ-induced spontaneous and evoked pain behaviors,and the up-regulation of P2X7R and IL-1β in the spinal cord.These data suggested that the up-regulation of P2X7Rs mediates microglial activation in the spinal dorsal horn,and therefore contributes to the development of BmK I-induced pain.