Electroporation-Mediated Immunization of a Candidate DNA Vaccine Expressing Dengue Virus Serotype 4 prM-E Antigen Confers Long-Term Protection in Mice

• 来源期刊: 中国病毒学
• 年，卷（期）: 2019,（1）
• 页码范围: 88-96
• 页数: 9页
• 语种: 英文

摘要

Dengue fever,caused by dengue viruses (DENVs),is a widespread mosquito-borne zoonotic disease;however,there is no available anti-dengue vaccine for worldwide use.In the current study,a DNA vaccine candidate (pV-D4ME) expressing prM-E protein of DENV serotype 4 (DENV-4) was constructed,and its immunogenicity and protection were evaluated in immunocompetent BALB/c mice.The pV-D4ME candidate vaccine induced effective humoral and cellular immunity of mice against DENV-4 in vivo when administered both at 50 μg and 5 μg through electroporation.Two weeks after receiving three immunizations,both doses of pV-D4ME DNA were shown to confer effective protection against lethal DENV-4 challenge.Notably,at 6 months after the three immunizations,50 μg,but not 5 μg,of pV-D4ME could provide stable protection (100％ survival rate) against DENV-4 lethal challenge without any obvious clinical signs.These results suggest that immunization with 50 μg pV-D4ME through electroporation could confer effective and long-term protection against DENV-4,offering a promising approach for development of a novel DNA vaccine against DENVs.