Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells

• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2019,（4）
• 页码范围: 673-687
• 页数: 15页
• 语种: 英文

摘要

Ras-related C3 botulinum toxin substrate 1 (Rac1),a member of the Rho GTPase family which plays important roles in dendritic spine morphology and plasticity,is a key regulator of cytoskeletai reorganization in dendrites and spines.Here,we investigated whether and how Rac1 modulates synaptic transmission in mouse retinal ganglion cells (RGCs) using selective conditional knockout of Rac1 (Rac1-cKO).Rac1-cKO significantly reduced the frequency of AMPA receptor-mediated miniature excitatory postsynaptic currents,while glycine/GABAA receptor-mediated miniature inhibitory postsynaptic currents were not affected.Although the total GluA1 protein level was increased in Rac1-cKO mice,its expression in the membrane component was unchanged.RaclcKO did not affect spine-like branch density in single dendrites,but significantly reduced the dendritic complexity,which resulted in a decrease in the total number of dendritic spine-like branches.These results suggest that Rac1 selectively affects excitatory synaptic transmission in RGCs by modulating dendritic complexity.