Measurement of Free and Glucuronidated Cardamonin in Rat Plasma and Bile Using UPLC-MS/MS and Its Application to a Pharmacokinetic and Bile Excretion Study

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（6）
• 页码范围: 962-966
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s40242-019-9275-2

摘要

A rapid and accurate ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-MS/MS) method was established and validated for the measurement of two forms of cardamonin,i.e.,free and glucuronidated,in the plasma and bile of rats.Cardamonin and an internal standard (1,8-dihydroxyanthraquinone) were extracted from plasma and bile with ethyl ether via liquid-liquid extraction.The analytes in the extracts were separated by an Agilent Zorbax Stable Bond-C18 colurnn(2.1 mm×50 mm,1.8 μm) under isocratic elution conditions[acetonitrile(A) and 0.1％ ammonium formate in water(B),40∶60(volume ratio)] with a flow rate of 0.4 mL/min,and mass spectrometry in negative ion MRM mode was implemented for analysis.Good linearity over the wide ranges of 0.01-5 μg/mL for plasma and 0.025-10 μg/mL for bile samples was acquired.Method validation was performed according to the FDA guidelines for bioanalytical methods.