A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(Ⅱ) Ions in HEPES Buffer Solution and Living Cells

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（6）
• 页码范围: 967-971
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s40242-019-9246-7

摘要

A highly sensitive fluorescent "turn-on" probe NapP-deap based on naphthalimide derivative was developed that bound Hg2+ ions rapidly in the N-2-hydroxyethylpiperazine-N-ethane-sulphonic acid(HEPES) buffer solution via photo-induced electron transfer(PET) being inhibited mechanism.The titration experiment displayed that the emission intensity of NapP-deap at 540 nm was almost linearly increased by about 3-fold.The Job's plot showed a stoichiometry factor of 1∶1 of the ligand-to-metal ratio.The detection limit of fluorescent probe was calculated to be 6.2×10-9 mol/L.1H NMR studies could confirm that one Hg2+ ion was bound by the N atoms(a,b) of piperazine or the N atom(c) ofpyridine.The fluorescent probe could be used for the detection of Hg2+ ions in living cells.