Synthesis and Photophysical Properties of Conjugates of Green Fluorescent Protein (GFP) Chromophore and 2'-Deoxy-Uridine Developed as Labelled Building Blocks for Oligonucleotide Probes

• 作者: Abed Saady Bilha Fischer
• 来源期刊: 药剂与药理学:英文版
• 学科: 医学
• 关键词: INTERCALATOR GFP 2'-deoxyuridine INTERCALATOR CONJUGATES
• 年，卷（期）: 2019,（9）
• 页码范围: 510-519
• 页数: 10页
• 分类号: R

摘要

Fluorescent probes with high signal/background ratio are needed for hybridization assays. Hence, the chromophore of green fluorescent protein (GFP), 4-hydroxybenzylidene-imidazolinone (HBI), was targeted as a potential fluorescent intercalator. For producing a building block for fluorescent oligonucleotide probes, 2'-deoxyuridine (dU) was conjugated with HBI via a flexible carbon-spacer. dUHBI conjugates highly fluoresce in glycerol (λem 460 nm,Φ 0.31), and are 109-fold more emissive than in methanol, implying the potential of dUHBI-labeled oligonucleotides as probes for hybridization assays.