Enhanced Bombyx genome editing via Cas9 ribonucleoprotein injection

• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2019,（6）
• 页码范围: 1059-1062
• 页数: 4页
• 语种: 英文
• DOI: 10.1111/1744-7917.12633

摘要

Dear Editor, The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has become a popular and powerful method for manipulating genomes with considerably little effort and high efficiency.It allows for the generation of targeted indels,genomic structure variations,and insertion of foreign fragments.However,increasing concerns have been raised about possible off-target effects.Off-target editing might cause unwanted disruptions at regions homologous to the single guide RNA (sgRNA).For application in entomology,off-target editing poses a big concern in the direct observation of mutant phenotypes in somatic tissues,because the sequence-phenotype causation may be confounded (Xu et al.,2015).In addition,it is of particular importance for research and field application of gene drive,an approach used for controlling invasive species and disease-carrying insect vectors (Taning et al.,2017).