Lab-in-cell based on spontaneous amino-yne click polymerization

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2019,（9）
• 页码范围: 1198-1203
• 页数: 6页
• 语种: 英文

摘要

Unnatural reaction in the living cells is a powerful tool for biological research.However,the polymerization inside cells is rarely reported.In this work,a lab-in-cell is illustrated based on our developed spontaneous amino-yne click polymerization.Carbonyl group activated terminal diyne can spontaneously polymerize with tetraphenylethene (TPE)-containing primary diamine inside cells,and polymer with weight-average molecular weight of 7,300 was yielded.By utilizing this in vivo amino-yne click polymerization and taking advantage of the aggregation-induced emission feature ofTPE,a "turn-on" cell imaging was realized,and in-situ killing of cells was also acquired by destroying the structures of actin and tubulin,which cannot be realized by preprepared polymer.This strategy provides a useful platform and holds great promise in biochemistry and therapy applications.