Nanopore-based DNA Supersandwich Structure for Detection of Streptavidin

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（5）
• 页码范围: 837-841
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s40242-019-8378-0

摘要

Natural and synthetic nanopores are increasingly popular tools in biosensors.In this work,the DNA supersandwich structure,which was made from two specially designed probes has been used to be fabricated in solid nanopores.Integrating the idea of affinity between streptavidin and biotin,the DNA supersandwich structure with biotins was successfully constructed for streptavidin detection,and the limitation of detection was found to be 10 frmol/L.This nanodevice allows specific,sensitive and versatile detection of diverse analytes with easy operations,thus we believe that it could be developed to detect some disease-related molecular targets and play a considerable role in biotechnology.