Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（3）
• 页码范围: 504-508
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s40242-019-8283-6

摘要

A novel artificial esterase based on chitosan and amino acids was synthesized in the present study.The Fmoc-His and Glu were linked to chitosan by active ester method(AEM).The hydroxide radical in chitosan,imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase.Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution.As for catalytic activity,chitosan-His-Glu was found to be more efficient than chitosan-His and chitosan-Glu in mimicking the core catalytic sites of natural esterase,and the best ratio(mass ratio) of chitosan-His-Glu∶CMCS was 1∶3.Ftrthermore,metal ions,such as Ca2+,Mg2+,Fe2+,etc.,were able to improve the catalytic efficiency of artificial esterase.And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.