DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（3）
• 页码范围: 514-520
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s40242-019-9007-7

摘要

To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-l(DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs),HA/PLGA microspheres with different HA content (10％,30％,50％,mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50％ HA were coated with IGF-1 and DOPA-IGF-1,respectively.They were co-cultured with rBMSCs,respctively.Cell counting kit-8(CCK-8) detection,confocal laser scanning microscopy(CLSM),alkaline phosphatase(ALP) detection and osteogenesis related genes COL1A1,Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity,cell morphology,differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups.The results showed that rBMSCs proliferation increased in an HA content dependent manner,and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups,in particular in DOPA-IGF-1 coated group,and the differences were more remarkable over time (P＜0.05).HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs,and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation ofrBMSCs.