Design of fibronectin type Ⅲ domains fused to an elastin-like polypeptide for the osteogenic differentiation of human mesenchymal stem cells

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: extracellular matrix proteins fibronectin elastin-like peptide inverse transition cycling osteogenic differentiation
• 年，卷（期）: 2019,（8）
• 页码范围: 856-863
• 页数: 8页
• 语种: 英文
• DOI: 10.1093/abbs/gmz063

摘要

Extracellular matrix (ECM) including fibronectin (FN) and elastin plays a pivotal role in providing a microenvironment to support tissue regeneration in stem cell therapy.To develop a novel biomimetic ECM for stem cell differentiation,we engineered FN type Ⅲ 9 and 10 domains fused to elastin-like polypeptides (FN-ELPs).The recombinant FN-ELP fusion protein was expressed in Escherichia coli and purified by inverse transition cycling.Human mesenchymal stem cells (hMSCs) cultured on plates coated with FN-ELP had significantly greater adhesion activity and proliferation than cells grown on non-coated plates.FN-ELP induced the osteogenic differentiation by elevating alkaline phosphatase (ALP) and mineralization activity of hMSCs.Furthermore,the osteogenic marker gene expressions of ALP,collagen type Ⅰ (Col Ⅰ),osteopontin (OPN),and transcriptional coactivator with a PDZ-binding motif (TAZ) were increased in hMSCs cultured on plates coated with FN-ELR We reported a novel biomimetic ECM with potential for bone regeneration that promotes the osteogenic differentiation of hMSCs.