A ratiometric fluorescent sensor for tracking Cu(Ⅰ) fluctuation in endoplasmic reticulum

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2019,（4）
• 页码范围: 465-474
• 页数: 10页
• 语种: 英文

摘要

A two-photon ratiometric fluorescent sensor for Cu+ in endoplasmic reticulum (ER),CNSB,was developed via coumarin/ASBD integration based on FRET mechanism.In solution,CNSB shows reversible,highly-specific ratiometric response to Cu+.Moreover,CNSB exhibits suitable Kd value,suggesting the possibility of detecting Cu+ in the living cells.The probe can enter the MCF-7 cells easily and specifically locates in the ER.The highly specific ratiometric response of CNSB toward Cu+ in MCF-7 cells provides the sensor the capacity to visualize both exogenous and endogenous Cu+ in the ER via fluorescence imaging.Next,CNSB was utilized to detect the fluctuation and distribution of Cu+ under ER stress in MCF-7 cells,which confirmed directly the relationship between Cu+ enhancement and ER stress.Meanwhile,the two-photon ability of coumarin facilitated the sensor to visualize Cu+ fluctuation via two-photon fluorescence imaging.In addition,the spatial distribution of Cu+ in the heart slice of the 14-day-old rat was demonstrated using CNSB.This study demonstrates the promising potential of CNSB in clarifying the Cu+-dependent signaling in the ER stress-related diseases.