Fluorescence imaging of Cu(Ⅰ) in endoplasmic reticulum of live cells and tissue

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2019,（7）
• 页码范围: 887-888
• 页数: 2页
• 语种: 英文

摘要

Copper (Cu) is an essential element to living organisms.The redox activity of Cu gives rise to redox cycling reactions between Cu2+ and Cu+.Organisms harness this redox capability for their biological functions by tightly regulating copper homeostasis in the processes of cellular uptake,intracellular transport,protein loading and storage [1].Abnormal levels and uncontrolled speciation of Cu2+/Cu+ in subcellular organelles can lead to various human diseases,such as Wilson's disease,Menkes disease,and neurodegenerative diseases [2].It is important to determine the concentration and speciation of Cu for understanding biological processes and for disease diagnosis.While many spectroscopic techniques can readily determine total concentration of Cu in biological specimens,it is more challenging to monitor the dynamic fluctuation of labile Cu+ in live cells.