Spectral Study on the Interactions Among Cu(Ⅱ),Doxorubicin and CopC

• 来源期刊: 高等学校化学研究（英文版）
• 年，卷（期）: 2019,（1）
• 页码范围: 53-59
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s40242-019-8284-5

摘要

Interactions among Cu(Ⅱ),doxorubicin and copper operon C(CopC) have been investigated in detail by means of fluorescence,UV-Vis,IR spectra,isothermal titration calorimetry(ITC) and molecular docking in Tris-HC1 buffer(50 mmol/L,pH=7.4,25 ℃).The results suggest that Cu(Ⅱ)-doxorubicin is formed in a Cu(Ⅱ) to doxorubicin molar ratio of 1:2,and the conditional stability constant,K[Cu(Ⅱ)-doxorubicin] is 1.90× 109 L2/mol2,CopC and doxorubicin can form a 1:1 complex,the conditional stability constant is greater than 105 L/mol.Binding of doxorubicin causes a conformational change in CopC with the reduction of β-sheet and increase of random coil,and the stability of CopC is decreased.Cu(Ⅱ),doxorubicin and CopC can form a CopC-Cu(Ⅱ)-doxorubicin ternary complex.The formation of CopC-Cu(Ⅱ)-doxorubicin reduced greatly the reduction rate of Cu(Ⅱ) by ascorbate(Vc),i.e.,the binding of doxorubicin affects the action of CopC as redox switch.