Dose-dependent effects of NMDA on retinal and optic nerve morphology in rats

• 作者: Aimy Abdullah Azliana Jusnida Ahmad Jafri Igor Iezhitsa Lidawani Lambuk Nafeeza Mohd Ismail Nor Salmah Bakar Puneet Agarwal Renu Agarwal
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: GLAUCOMA EXCITOTOXICITY N-methyl-Daspartate RETINA optic nerve DOSE-DEPENDENT EFFECTS
• 年，卷（期）: gjykzzywb_2019,（5）
• 页码范围: 746-753
• 页数: 8页
• 分类号: R775

摘要

AIM: To investigate dose-dependent effects of N-methylD-aspartate(NMDA) on retinal and optic nerve morphology in rats.METHODS: Sprague Dawley rats, 180-250 g in weight were divided into four groups. Groups 1, 2, 3 and 4 were intravitreally administered with vehicle and NMDA at the doses 80, 160 and 320 nmol respectively. Seven days after injection, rats were euthanized, and their eyes were taken for optic nerve toluidine blue and retinal hematoxylin and eosin stainings. The TUNEL assay was done for detecting apoptotic cells.RESULTS: All groups treated with NMDA showed significantly reduced ganglion cell layer(GCL) thickness within inner retina, as compared to control group. Group NMDA 160 nmol showed a significantly greater GCL thickness than the group NMDA 320 nmol. Administration of NMDA also resulted in a dose-dependent decrease in the number of nuclei both per 100 μm GCL length and per 100 μm2 of GCL. Intravitreal NMDA injection caused dosedependent damage to the optic nerve. The degeneration of nerve fibres with increased clearing of cytoplasm was observed more prominently as the NMDA dose increased. In accordance with the results of retinal morphometry analysis and optic nerve grading, TUNEL staining demonstrated NMDA-induced excitotoxic retinal injury in a dose-dependent manner.CONCLUSION: Our results demonstrate dose-dependent effects of NMDA on retinal and optic nerve morphology in rats that may be attributed to differences in the severity of excitotoxicity and oxidative stress. Our results also suggest that care should be taken while making dose selections experimentally so that the choice might best uphold study objectives.