Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model

• 作者: ?z Ahmi Güne?Alime Naz?ro?lu Mustafa T?k Levent U?uz Cihangir Yal??n T?k?zlem Yi?it Musa
• 来源期刊: 国际眼科杂志:英文版
• 学科: 医学
• 关键词: APOPTOSIS ARPE-19 cell ASTAXANTHIN OXIDATIVE stress
• 年，卷（期）: gjykzzywb_2019,（6）
• 页码范围: 930-935
• 页数: 6页
• 分类号: R77

摘要

AIM: To observe the protective effect of astaxanthin(AST) against hydroquinone(HQ) mediated cell death in the apoptotic cascade and evaluate intracellular Ca2+ release, caspase-3, and-9 activation, reactive oxygen species(ROS) production in ARPE-19 cells.METHODS: We cultured ARPE-19 cells in special mediums and performed MTT tests to determine protective effect of AST, before exposing the cells to HQ in an incubator. We analyzed intracellular Ca2+ release experiments, mitochondrial membrane depolarization, glutathione(GSH), glutathione peroxidase(GSH-Px) and ROS experiments, and apoptosis assay.RESULTS: ROS production ranges depend on the amount of cell death. We computed the correlation between ROS ranges and cell death by 20,70-dichlorofluorescein fluorescence, and Ca2+ levels by Fura-2-AM. HQ-induced cell death found out to rise ranges of caspase-3 and-9, and mitochondrial depolarization. These three steps were delayed by AST management.CONCLUSION: ARPE-19 cells are avoided from HQinduced ROS production and caspase-3 and-9 activation by AST. AST may limit the range of caspase synthesis, Ca2+ release and excess production of ROS with antiapoptotic effect. This study proposes a new therapeutic approach for the treatment of age-related macular degeneration.