Establishment of Tissue Culture Regeneration System in Bama Hemp (Cannabis sativa L.)

• 作者: Desheng ZHU Jian LI Yanfeng NONG
• 来源期刊: 农业生物技术:英文版
• 学科: 工学
• 关键词: HEMP SEEDS TISSUE culture REGENERATION system
• 年，卷（期）: 2019,（6）
• 页码范围: 1-3
• 页数: 3页
• 分类号: TN0

摘要

[Objectives]This study was conducted to establish a tissue culture regeneration system in Bama hemp(Cannabis sativa L.).[Methods]Using hemp seeds as explants,a regeneration system was established through explant sterilization,callus induction,callus differentiation,and rooting culture.[Results]The results showed that the best sterilization effect was achieved when sterilizing with 75%ethanol for 30 s,followed by 0.1%HgCl 2 solution for 9 min,with a contamination rate as low as 11.4%.In presence of 3 mg/L 2,4-D and 0.1 mg/L6-BA,the callus induction effect from hemp seeds was better.The formula for better differentiation of callus was MS+2.0 mg/L 6-BA+0.2 mg/L NAA.IBA had a promoting effect on the rooting of hemp aseptic plantlets.The highest rooting rate reached 80%when MS+0.3 mg/L IBA were used.[Conclusions]This study established a hemp seed regeneration system to provide technical support for the conservation and breeding of hemp germplasm resources.