Self-assembled sialyllactosyl probes with aggregation-enhanced properties for ratiometric detection and blocking of influenza viruses

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2019,（24）
• 页码范围: 1902-1909
• 页数: 8页
• 语种: 英文

摘要

Infection and dissemination of influenza viruses (Ⅳs) causes serious health concerns worldwide.However,effective tools for the accurate detection and blocking of Ⅳs remain elusive.Here,we develop a new sialyllactosyl probe with self-assembled core-shell structure for the ratiometric detection and blocking of Ⅳs.N,N'-diaryl-dihydrodibenzo[a,c]phenazines were used to form the core structure by hydrophobic assembly in an aqueous solution with an aggregation-enhanced blue fluorescence mission.Subsequently,dicyanomethylene-4H-pyran-based sialyllactosides were used for self-assembly with the core structure,producing the sialyllactosyl probe that emits a red fluorescence due to F(o)rster resonance energy transfer.The probe developed has been proven to be available for (1) the fluorescence ratiometric detection of Ⅳs through selective interaction with the sialyllactosyl-binding proteins on the virus surface,and (2) effectively blocking the invasion of human-infecting Ⅳs towards host cells as accentuated by the sialyllactosides on the surface of the probes.