Dual roles of juvenile hormone signaling during early oogenesis in Drosophila

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: cap cells germline stem cells juvenile hormone oogenesis ovary
• 年，卷（期）: 2020,（4）
• 页码范围: 665-674
• 页数: 10页
• 语种: 英文
• DOI: 10.1111/1744-7917.12698

摘要

Juvenile hormone (JH) signaling plays crucial roles in insect metamorphosis and reproduction.Function of JH signaling in germline stem cells (GSCs) remains largely unknown.Here,we found that the number of GSCs significantly declined in the ovaries of Met,Gce and JHAMT mutants.Then we inhibited JH signaling in selected cell types of ovaries by expressing Met and Gce or Kr-h1 double-stranded RNAs (dsRNAs) using different Gal4 drivers.Blocking of JH signaling in muscle cells has no effect on GSC numbers.Blocking of JH signaling in cap cells reduced GSCs cells.Inductive expression of Met and Gce dsRNA but not Kr-h1 by Nos-Gal4 increased GSC cells.These results indicate that JH signaling plays an important role in GSC maintenance.