P2X7 receptor antagonist recovers ileum myenteric neurons after experimental ulcerative colitis

• 作者: Cristina Eusébio Mendes Mariá Munhoz Evangelinellis Marta Righetti Múcio Cevulla Silva Lourenco Patricia Castelucci Roberta Figueiroa Souza
• 来源期刊: 世界胃肠病理生理学杂志:英文版(电子版)
• 学科: 医学
• 关键词: P2X7 receptor Brilliant blue G Myenteric plexus Experimental ulcerative colitis ILEUM Chemical coding
• 年，卷（期）: 2020,（4）
• 页码范围: 84-103
• 页数: 20页
• 分类号: R574.62

摘要

BACKGROUND The P2X7 receptor is expressed by enteric neurons and enteric glial cells.Studies have demonstrated that administration of a P2X7 receptor antagonist,brilliant blue G(BBG),prevents neuronal loss.AIM To report the effects of BBG in ileum enteric neurons immunoreactive(ir)following experimental ulcerative colitis in Rattus norvegicus albinus.METHODS 2,4,6-trinitrobenzene sulfonic acid(TNBS group,n=5)was injected into the distal colon.BBG(50 mg/kg,BBG group,n=5)or vehicle(sham group,n=5)was given subcutaneously 1 h after TNBS.The animals were euthanized after 24 h,and the ileum was removed.Immunohistochemistry was performed on the myenteric plexus to evaluate immunoreactivity for P2X7 receptor,neuronal nitric oxide synthase(nNOS),choline acetyltransferase(ChAT),HuC/D and glial fibrillary acidic protein.RESULTS The numbers of nNOS-,ChAT-,HuC/D-ir neurons and glial fibrillary acidic protein-ir glial cells were decreased in the TNBS group and recovered in the BBG group.The neuronal profile area(μm~2)demonstrated that nNOS-ir neurons decreased in the TNBS group and recovered in the BBG group.There were no differences in the profile areas of ChAT-and HuC/D-ir neurons.CONCLUSION Our data conclude that ileum myenteric neurons and glial cells were affected by ulcerative colitis and that treatment with BBG had a neuroprotective effect.Thus,these results demonstrate that the P2X7 receptor may be an important target in therapeutic strategies.