Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2020,（1）
• 页码范围: 46-53
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11655-019-3071-1

摘要

Objective:To evaluate the effects of combination of Radix Astragali (RA) and Radix Salviae Miltiorrhizae (RS) on kidney of spontaneously hypertensive rats (SHRs) and renal intrinsic cells.Methods:SHRs were intragastrically administrated with RA (5.09 g/kg) and RS (2.55 g/kg) either alone or with combination for 4 weeks;valsartan (13.35 mg/kg) was used as a positive control.Blood pressure and renal ultrasonography were monitored periodically.The biomarkers [microalbumin (mALB),cystatin C,angiotensin Ⅱ (Ang Ⅱ),interleukin-1 beta (IL-1β),and β 2-microglobulin (β 2-Mg),etc.] in serum and urine were measured by enzyme-linked immunosorbent assay (EMSA).The protein expressions [phosphorylated adenosine 5'-monophosphate-activated protein kinase-α1 (p-AMPKα1),sestrin-β,calcium/calmodulin-dependent protein Iinase kinase-β (CaMKK-β),phosphoinositide 3-kinases (PI3K),serine-threonine protein kinase 1 (AKT1),and vascular endothelial growth factor receptor 2 (VEGFR2)] in renal cortex were determined by Western blot.In vitro,the hypertensive cellular model was established by applying 2 x 10-6 mol/L Ang Ⅱ.The primary human podocytes,human glomerular endothelial cells (HRGECs),and human proximal tubular epithelial cells (HK-2s) were pre-incubated with sulfotanshinone sodium (Tan,10 μ g/mL) and/or calycosin-7-O-β-D-glucoside (Cal,5 μ g/mL).The cellular viability and apoptosis were assayed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Annexin V/PI staining,respectively.The level of endothelial nitric oxide synthase (eNOS) in culture supernatant was determined by ELISA.Results:RA+RS significantly decreased the diastolic blood pressure,renal vascular resistance index,and parenchymal thickness,increased 24 h urinary volume as well as lowered the levels of urine mALB and serum cystatin C,IL-1 lβ and β 2-Mg of SHRs (P＜0.05 vs.SHRs).The decreased protein levels of p-AMPKα 1,sestrin β and CaMKK-β and the increased protein levels of PI3K,AKT1 and VEGFR2 in renal cortex of SHRs were normalized after RA+RS treatment (P＜0.05).In vftro,Tan and Cal attenuated the Ang Ⅱ-induced abnormal proliferation and increased the apoptosis of HRGECs and HK-2s and improved the level of eNOS in culture supematant.Whereas,neither of them showed powerful effect on podocyte.Conclusion:The combination of RA and RS had potential effects on alleviating the renal damages of SHRs and the renoprotection was independent of blood pressure level.