Mouthwash with Active Oxygen (blue<sup>®</sup>m) Induces Keratinocytes Proliferation

• 作者: Bruna Marca Mattei Grasieli de Oliveira Ramos Paloma Santos de Campos Soraia A. W. Imanishi Suyany Gabriely Weiss Tatiana Miranda Deliberador
• 来源期刊: 口腔学期刊(英文)
• 学科: 数学
• 关键词: KERATINOCYTES Cell Proliferation Oxygen
• 年，卷（期）: 2020,（6）
• 页码范围: 107-114
• 页数: 8页
• 分类号: O17

摘要

<span>Chlorhexidine is widely used in dentistry to treat various gingival conditions. Its side effects are widely described. Histologically, the gingiva consists of a stratified squamous epithelium, with a predominance of keratinocytes, the latter being fundamental in healing processes. Thus, the objective of this </span><i><span>in vitro</span></i><span><span> study was to evaluate the effects of a new product with active oxygen (blue^&#174;</span><span>m) on keratinocytes. Keratinocytes (HACAT) were incubated with different concentrations of blue</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">^{<span style="font-size:10px;white-space:normal;">&#174;</span><span style="font-size:10px;white-space:normal;"></span>}</span></span></span></span></span></span></span></span></span><span>m (1, 10 and 100 μl/ml), and another well was used as a control, without the presence of mouthwash. After 24, 48 and 72 hours, cell proliferation was analyzed by CyQUANT</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><sup style="vertical-align:super;"><span style="white-space:nowrap;">^{<span style="font-size:10px;white-space:normal;">&#174;</span><span style="font-size:10px;white-space:normal;"></span>}</span></sup></span></span></span></span></span></span></span></span><span><span><span>. It was possible to observe that lower concentrations (1 μl/ml) of blue</span><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:norm