Establishment of Recombinase Polymerase Amplification for Rapid Detection of Spring Viremia of Carp Virus(SVCV)

• 作者: Duan Xiaohui Liang Junni Lin Sen Liu Ning Shang Di Yin Weili
• 来源期刊: 动物与饲料科学:英文版
• 学科: 农学
• 关键词: Recombinase polymerase amplification Rapid detection Spring viremia of carp virus(SVCV)
• 年，卷（期）: 2020,（2）
• 页码范围: 29-32
• 页数: 4页
• 分类号: S94

摘要

[Objective]The paper was to develop a rapid method for the detection of spring Viremia of carp virus(SVCV).[Method]The specific primers were designed by targeting the G gene of SVCV.The recombinase polymerase amplification(RPA)assay for detecting SVCV was estab-lished by optimizing the reaction conditions.The optimal amplification temperature of RPA assay was 30℃,and the test could be finished within 20 min.[Result]The method was specific with no cross-reaction with other common fish infectious viruses.Sensitivity test showed that the lowest detection limit of the method was 89.2 copies/μL,higher than that of traditional RT-PCR.Moreover,a total of 80 clinical samples were detected by RPA and RT-PCR,respectively.The weak positive samples tested by RT-PCR could be detectable with RPA,indicating that RPA assay could be used in clinical detection.[Conclusion]The method established is rapid,simple,specific and sensitive for testing SVCV,and it will be widely used in grassroots laboratory and on-site inspection.