Cu2+ reduces hemolytic activity of the antimicrobial peptide HMPI and enhances its trypsin resistance

• 作者: Ahmed Shabbir Chaudhry Fengjing Jia Jiayi Wang Jinxiu Peng Kairong Wang Peihan Xu Ping Zhao Shuai Qiu Wenjin Yan Xiaolei Liang Yang Yang Zhaoqing Xu
• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: antimicrobial peptide HMPI antifungal activity protease stability trypsin
• 年，卷（期）: 2020,（6）
• 页码范围: 603-611
• 页数: 9页
• 语种: 英文
• DOI: 10.1093/abbs/gmaa043

摘要

Nowadays,drug-resistant microbes are becoming a serious clinical problem threatening people's health and life.Antimicrobial peptides (AMPs) are believed to be potential alternatives of conventional antibiotics to combat the threat of drug-resistant microbes.However,the susceptibility of AMPs toward proteases is one of the major problems limiting their clinical use.In the present study,we reported the effect of Cu2+ on the bioactivity of AMP HMPI.We found that the addition of Cu2+ could improve the protease resistance of AMP HMPI without affecting its bioactivity.Notably,after the binding of Cu2+ with HMPI,the hemolytic activity of HMPI was greatly decreased.In addition,our results also demonstrated that the addition of Cu2+ increased the production of reactive oxygen species in the fungal cells,which may be a supplement for the antifungal activity of HMPI.In conclusion,the introduction of Cu2+ may provide an inorganic strategy to improve the stability and decrease the hemolytic activity of AMP HMPI,while maintaining its antifungal activity.