Pentadecapeptide BPC 157 resolves Pringle maneuver in rats,both ischemia and reperfusion

• 作者: Alenka Boban Blagaic Andreja Petrovic Antonija Duzel Antonio Kokot Borna Vrdoljak Domagoj Drmic Dominik Malekinusic Ivan Barisic Ivan Krezic Ivan Mirkovic Katarina Horvat Pavlov Katarina Kasnik Kovac Marijan Kolovrat Mario Knezevic Predrag Sikiric Slaven Gojkovic Sven Seiwerth Tamara Kralj
• 来源期刊: 世界肝病学杂志:英文版(电子版)
• 学科: 医学
• 关键词: BPC 157 Pringle maneuver RATS Portal hypertension Caval hypertension ISCHEMIA
• 年，卷（期）: 2020,（5）
• 页码范围: 184-206
• 页数: 23页
• 分类号: R57

摘要

BACKGROUND The Pringle maneuver[portal triad obstruction(PTO)]provides huge disturbances during ischemia and even more thereafter in reperfusion.Contrarily,a possible solution may be stable gastric pentadecapeptide BPC 157,with already documented beneficial effects in ischemia/reperfusion conditions.Recently,BPC 157,as a cytoprotective agent,successfully resolved vessel occlusions in rats(ischemic colitis;deep vein thrombosis,superior anterior pancreaticoduodenal vein;bile duct cirrhosis)through rapid collateral vessel recruitment to circumvent vessel occlusion.Thereby,medication BPC 157 regimens were administered as a single challenge before and during ischemia or,alternatively,at various time points during reperfusion.AIM To introduce BPC 157 therapy against pringle maneuver-damage.METHODS In deeply anesthetised rats,the portal triad was clamped up for 30 min.Rats then underwent reperfusion for either 15 min or 24 h.Medication[(10μg,10 ng/kg)regimens,administered as a single challenge]picked(a)ischemia,PTO period[at 5 min before(ip)or at 5 or 30 min of ligation time(as a bath to PTO)]or(b)reperfusion,post-PTO period[at 1 or 15 min(bath during surgery)or 24 h(ip)reperfusion-time].We provided gross,microscopy,malondialdehyde,serum enzymes,electrocardiogram,portal,caval,and aortal pressure,thrombosis and venography assessments.RESULTS BPC 157 counteracts electrocardiogram disturbances(increased P wave amplitude,S1Q3T3 QRS pattern and tachycardia).Rapidly presented vascular pathway(portal vein-superior mesenteric vein-inferior mesenteric vein-rectal veins-left ileal vein-inferior caval vein)as the adequate shunting immediately affected disturbed haemodynamics.Portal hypertension and severe aortal hypotension during PTO,as well as portal and caval hypertension and mild aortal hypotension in reperfusion and refractory ascites formation were markedly attenuated(during PTO)or completely abrogated(reperfusion);thrombosis in portal vein tributaries and inferior caval vein or hepatic artery was counteracted during portal