Synchrotron Radiation-Based Three-Dimensional Visualization of Angioarchitectural Remodeling in Hippocampus of Epileptic Rats

• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2020,（4）
• 页码范围: 333-345
• 页数: 13页
• 语种: 英文

摘要

Characterizing the three-dimensional (3D) morphological alterations of microvessels under both normal and seizure conditions is crucial for a better understanding of epilepsy.However,conventional imaging techniques cannot detect microvessels on micron/sub-micron scales without angiography.In this study,synchrotron radiation (SR)-based X-ray in-line phase-contrast imaging (ILPCI) and quantitative 3D characterization were used to acquire high-resolution,high-contrast images of rat brain tissue under both normal and seizure conditions.The number of blood microvessels was markedly increased on days 1 and 14,but decreased on day 60 after seizures.The surface area,diameter distribution,mean tortuosity,and number of bifurcations and network segments also showed similar trends.These pathological changes were confirmed by histological tests.Thus,SR-based ILPCI provides systematic and detailed views of cerebrovascular anatomy at the micron level without using contrast-enhancing agents.This holds considerable promise for better diagnosis and understanding of the pathogenesis and development of epilepsy.