Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats

• 作者: Chuanwu Cao Hui Zhao Maoquan Li Rongfeng Shi Shilong Han Suming Zhao Weishuai Lian Xiaohu Yang Yinpeng Jin
• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: human umbilical cord mesenchymal stem cells diabetic foot ulcer tissue repair
• 年，卷（期）: 2020,（6）
• 页码范围: 620-630
• 页数: 11页
• 语种: 英文
• DOI: 10.1093/abbs/gmaa039

摘要

Diabetic foot ulcer (DFU) is one of diabetic complications,which is frequently present and tormented in diabetes mellitus.Most multipotent mesenchymal stromal cells (MSCs) are capable of immune evasion,providing an allogeneic,ready-to-use,cell product option for therapeutic applications.The beneficial effect of MSCs for the treatment of a variety of traumatic injuries,such as open wounds,has been extensively explored.In this study,a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells (hUC-MSCs) after intravenous transplantation and its role in DFU healing,so as to evaluate the potential of hUC-MSCs in the treatment of DFU.The diabetic rat model was established by streptozotocin injection,which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor.The hUC-MSCs were transplanted through femoral vein,and the ulcer cicatrization situation and the fate of hUC-MSCs were evaluated.Our data suggest that intravenously transplantated hUC-MSCs have the ability to migrate and locate to the wound tissue and are helpful to wound healing in DFU rats,partly by regulating inflammation,trans-differentiation and providing growth factors that promote angiogenesis,cell proliferation and collagen deposition.Herein,we demonstrate that hUC-MSC transplantation is able to accelerate DFU healing in rats and transplantation of exogenous stem cells may be a potential strategy for clinical application in DFUs.