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Accurate species-level identifications underpin many aspects of basic and applied biology;however,identifications can be hampered by a lack of discriminating morphological characters,taxonomic expertise or time.Molecular approaches,such as DNA "barcoding" of the cytochrome c oxidase (COI) gene,are argued to overcome these issues.However,nuclear encoding ofmitochondrial genes (numts) and poor amplification success of suboptimally preserved specimens can lead to erroneous identifications.One insect group for which these molecular and morphological problems are significant are the dacine fruit flies (Diptera:Tephritidae:Dacini).We addressed these issues associated with COI barcoding in the dacines by first assessing several "universal" COI primers against public mitochondrial genome and numt sequences for dacine taxa.We then modified a set of four primers that more closely matched true dacine COI sequence and amplified two overlapping portions of the COI barcode region.Our new primers were tested alongside universal primers on a selection of dacine species,including both fresh preserved and decades-old dry specimens.Additionally,Bactrocera tryoni mitochondrial and nuclear genomes were compared to identify putative numts.Four numt clades were identified,three of which were amplified using existing universal primers.In contrast,our new primers preferentially amplified the "true" mitochondrial COI barcode in all dacine species tested.The new primers also successfully amplified partial barcodes from dry specimens for which full length barcodes were unobtainable.Thus we recommend these new primers be incorporated into the suites of primers used by diagnosticians and quarantine labs for the accurate identification of dacine species.
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篇名 Development of internal COI primers to improve and extend barcoding of fruit flies (Diptera: Tephritidae: Dacini)
来源期刊 中国昆虫科学(英文版) 学科
关键词 Bactrocera biosecurity Dacus minibarcodes pseudogene Zeugodacus
年,卷(期) 2020,(1) 所属期刊栏目
研究方向 页码范围 143-158
页数 16页 分类号
字数 语种 英文
DOI 10.1111/1744-7917.12612
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研究主题发展历程
节点文献
Bactrocera
biosecurity
Dacus
minibarcodes
pseudogene
Zeugodacus
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
中国昆虫科学(英文版)
双月刊
1672-9609
11-3386/Q
北京北辰西路1号院
eng
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1404
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