Enhanced Production of Natural Carotenoids from Genetically Engineered Rhodobacter sphaeroides Overexpressing CrtA

• 作者: Hongfan CHEN Jiahui DU Jiamin ZHANG Lili JI Wei WANG Zhiping ZHAO
• 来源期刊: 农业生物技术:英文版
• 学科: 经济
• 关键词: Carotenoids CRTA RHODOBACTER sphaeroids FERMENTATION OVEREXPRESSION Optimization
• 年，卷（期）: 2020,（2）
• 页码范围: 1-4
• 页数: 4页
• 分类号: F42

摘要

Carotenoids act as precursors of vitamin A,antioxidants,enhancers of immunity,and are thus widely used in food and pharmaceutical industry.Microbial fermentation is one of the most important solutions for production of natural carotenoids.Rhodobacter sphaeroides is one of most promising bacteria employed for large scale production of carotenoids.In the present study,crtA located in the carotenoids biosynthesis pathway in R.sphaeroides was amplified by PCR.The overexpression vector pRKcrtA was constructed and subsequently transferred into R.sphaeroides,producing the genetically engineered strain R.sphaeroides 2.4.1/pRKcrtA overexpressing crtA.The carotenoid production from the genetically engineered strain was significantly increased.Fermentation procedure was optimized for further enhanced carotenoids production.