MiR-122-5p suppresses the proliferation, migration, and invasion of gastric cancer cells by targeting LYN

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: gastric cancer miR-122-5p LYN migration invasion
• 年，卷（期）: 2020,（1）
• 页码范围: 49-57
• 页数: 9页
• 语种: 英文
• DOI: 10.1093/abbs/gmz141

摘要

Gastric cancer (GC) is one of malignant tumors with high mortality and morbidity in the world.MicroRNA-122 (miR-122) acts as a tumor suppressor in a variety of cancers and has been found to be dominant in gastric adenocarcinoma.However,the specific biological function of miR-122-5p in GC is not completely clear.In this study,we found that miR-122-5p was low-expressed in GC tissues and cell lines by using qRT-PCR.Overexpression of miR-122-5p inhibited the proliferation,migration,and invasion of GC cells by using CCK-8 and transwell assays.On the contrary,downregulation of miR-122-5p promoted the proliferation,migration,and invasion of GC cells.In addition,we found that the expression of LYN,an Src family tyrosine kinase,was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis,immunohistochemistry,and qRT-PCR assays.Meanwhile,luciferase assay results indicated that LYN is a direct target of miR-122-5p in GC cells.Moreover,silencing LYN expression by its siRNA inhibited the proliferation,migration,and invasion of GC cells.Importantly,overexpression of LYN restored miR-122-5p-mediated inhibition of the proliferation,migration,and invasion of GC cells.Taken together,our results indicated miR-122-5p inhibited the proliferation,migration,and invasion by targeting LYN in GC.