[Objectives]This study was conducted to discuss the effects of tillering law and its controlling factors on the growth of Ophiopogon japonicus cv.[Methods]With O.japonicus cv as an experimental material,its average number of tillers,proliferation coefficient,total number of tillers,death number,germination index,germination rate,fresh root weight,root volume and survival rate were determined.[Results] The reproductive ability of the NAA treatments was significantly higher than that of the 6-BA treatments,and the average tillering capacity of the 5 mg/L NAA treatment was significantly higher,90% higher than that of the CK.The proliferation coefficient of O.japonicus cv was significantly better in the NAA treatments than in the 6-BA treatments,and the 5 mg/L-NAA treatment was more significant.The NAA treatments showed total numbers of tillers significantly higher than those of the 6-BA treatments,and exhibited significantly reduced deaths.The 1 mg/L NAA and 10 mg/L 6-BA experimental groups had the strongest germination-promoting effects on O.japonicus cv,which were significantly different from those of other experimental groups,and were 92% and 95% higher than that of the CK,respectively.As to the germination rate indicator of O.japonicus cv,the 1 mg/L NAA and 10 mg/L 6-BA experimental groups exhibited the highest values,which increased by 48% and 43%,respectively,compared with the CK.The 6-BA treatments significantly improved the root growth of O.japonicus cv compared with the NAA treatments,and they significantly promoted root volume of O.japonicus cv.The survival rate of O.japonicus cv treated with hormone NAA was 23.3% higher than that of the 6-BA treatment group.[Conclusions] This study provide a theoretical basis for improving the reproductive ability of O.japonicus cv and expanding its commercial production.