Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing

• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2020,（3）
• 页码范围: 202-216
• 页数: 15页
• 语种: 英文

摘要

Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain.The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2C glycoprotein (SAD-RV(△G)-N2C(G)) shows strong neuro-tropism in cell culture,but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2C(G) and two other widely-used retrograde tracers,SAD-RV(△G)-B19(G) and rAAV2-retro.We found that SAD-RV(△G)-N2C(G) enhanced the infection efficiency of long-projecting neurons by ～ 10 times but with very similar neuro-tropism,compared with SAD-RV(△G)-B19(G).On the other hand,SAD-RV(△G)-N2C(G) had an infection efficiency comparable with rAAV2-retro,but a more restricted diffusion range,and broader tropism to different types and regions of long-projecting neuronal populations.These results demonstrate that SAD-RV(△G)-N2C(G) can serve as an effective retrograde vector for studying neuronal circuits.