Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2020,（2）
• 页码范围: 130-137
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11655-019-3204-6

摘要

Objective:To determine the effects of Hydrangeae Dulcis Folium (EHDF) on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish (Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test:4 fish per group] or 6 groups (behavioural test:10-12 fish per group,whole-body cortisol:4 fish per group).Net handling stress (NHS) was used to induce physical stress.Fish were treated with vehicle or EHDF (5-20 mg/L) for 6 min before they were exposed to stress.And then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and locomotion.In addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH (0.2 IU/g,i.p.) challenge test was performed.Results:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5-20 mg/L (P＜0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS (P＜0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5-20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (P＜0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (P＜0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.