Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex

• 作者: Chao Zeng Chuangye Yan Gaoxingyu Huang Guangwen Yang Jianlin Lei Kunpeng Wang Minhao Liu Qiang Zhou Qifan Wang Qinghua Tao Sai Li Wusheng Zhang Xuechen Zhu Yanqing Zhang Yigong Shi
• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2020,（6）
• 页码范围: 532-540
• 页数: 9页
• 语种: 英文

摘要

The nuclear pore complex (NPC) mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells.Here we report the cryo-electron tomography (cryo-ET) structure of the luminal ring (LR) of the NPC from Xenopus laevis oocyte.The observed key structural features of the LR are independently confirmed by single-particle cnyo-electron microscopy (cryo-EM) analysis.The LR comprises eight butterfly-shaped subunits,each containing two symmetric wings.Each wing consists of four elongated,tubular protomers.Within the LR subunit,the eight protomers form a Finger domain,which directly contacts the fusion between the inner and outer nuclear membranes and a Grid domain,which serves as a rigid base for the Finger domain.Two neighboring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain,which displays two major conformations and appears to cushion neighboring NPCs.Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC.