First structural insights into CRISPR-Cas-guided DNA transposition

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2020,（3）
• 页码范围: 193-194
• 页数: 2页
• 语种: 英文

摘要

The transposition mechanism of the Vibrio cholerae Tn6677 transposon (VcTn6677) is based on the unique synergy between a classical transposition machinery (TnsABC-TniQ complex) and a nuclease-deficient type Ⅰ-F CRISPR-associated complex for antiviral defense (VcCascade).Four independent studies,three of which appeared in Cell Research,recently reported structures of the VcCascade,VcCascade-TniQ,and/or VcCascade-TniQ-dsDNA complexes as well as a TniQ dimer complex,providing the first insights into the assembly and DNA targeting mechanism of a CRISPR-associated transposase complex.