Isolation, Screening and Molecular Characterization of Multifunctional Plant Growth Promoting Rhizobacteria for a Sustainable Agriculture

• 作者: Aye Aye Khai Kay Thi Oo Pengcheng Fu Theint Theint Win
• 来源期刊: 美国植物学期刊(英文)
• 学科: 农学
• 关键词: PGPR RHIZOBACTERIA Maize Green-Gram SIDEROPHORE IAA
• 年，卷（期）: 2020,（6）
• 页码范围: 773-792
• 页数: 20页
• 分类号: S51

摘要

The use of PGPR as a multifunctional biofertilizer or biostimulant is an alternative way to prevent soil pollution and preserve agricultural for sustainable economy. In this study, 102 bacterial strains were isolated from rhizospheric soil of different crop fields. Among them, 15 bacterial isolates rich of NPK were selected to screen for PGP activity. It was found that 4 out 15 isolates were able to fix atmospheric nitrogen, 14 could solubilize phosphate and 5 could solubilize potassium. They were further examined for the production of hydrolytic enzymes (amylase, cellulose, chitinase, etc.), plant hormone (IAA) and plant defense substances (HCN, siderophore, etc.). All PGPR isolates were able to produce IAA, siderophore and ammonia while 2 isolates could produce HCN. Among them, 73.33% of selected isolates produced amylase, 80% produced cellulase, 66.67% produced pectinase, 93.33% produced chitinas</span><span><span style="font-family:Verdana;">e and </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-glucanase. For salt stress tolerance, all the isolates grew well in 5% NaCl while only 4 tolerated 9% NaCl. Among all isolates, 2 have antifungal activity and 5 have antibacterial activity. The best 6 isolates and consortium were tested to promote plant growth in green-gram and maize germination. Seed germination of green-gram and maize was observed the best in </span><i><span style="font-family:Verdana;">Acromobacter</span></i><span style="font-family:Verdana;"> <i>insolitus</i></span><span style="font-family:Verdana;"> S3 compared with other treatments. </span><i><span style="font-family:Verdana;">Pseudomonas</span></i><span style="font-family:Verdana;"> <i>plecoglossicida</i> </span><span style="font-family:Verdana;">B3 was found the best in fresh weight for bot</span></span><span style="font-family:Verdana;">h crops. The highest root formation was observed in </span><i><span style="font-family:Verdana;">Acromobacter</span></i><span style="font-family:Verdana;"> <i>insol