miR-487a performs oncogenic functions in osteosarcoma by targeting BTG2 mRNA

• 作者: Bo Mao Guoxing Xu Shaokun Wu Shoujun Zhao Wei Wu Zhiqian Gu
• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: osteosarcoma miR-487a BTG2 proliferation invasion
• 年，卷（期）: 2020,（6）
• 页码范围: 631-637
• 页数: 7页
• 语种: 英文
• DOI: 10.1093/abbs/gmaa034

摘要

Aberrant microRNA (miRNA) expression plays a critical role in osteosarcoma (OS) pathogenesis.In this study,we elucidated the involvement of miR-487a in OS and the underlying molecular mechanisms.We found that miR-487a was upregulated in OS clinical samples and cell lines.Knockdown of miR-487a suppressed OS cell growth and invasion and induced apoptosis;however,overexpression of miR-487a promoted OS cell growth and invasion.Accordingly,downregulation of miR-487a significantly suppressed tumor growth of OS xenografts in vivo.Furthermore,B-cell translocation gene 2 (BTG2) mRNA was found to be a novel target of miR-487a.Knockdown of BTG2 using small interfering RNA (siRNA) recapitulated the oncogenic effects of miR-487a,whereas BTG2 overexpression partially reversed these effects.Finally,miR-487a levels were found to be negatively correlated with BTG2 expression in OS clinical samples.Collectively,our data suggest that miR-487a is an oncogenic miRNA in OS and it lowers BTG2 expression.