TfR1 Extensively Regulates the Expression of Genes Associated with Ion Transport and Immunity

• 作者: Lei-Lei ZHAN Nan HUANG Qi WANG Wen-jing LI Xiao-long WANG Ya-xun WEI Yi CHENG
• 来源期刊: 当代医学科学(英文)
• 学科: 医学
• 关键词: transferrin receptor 1 OVEREXPRESSION RNA-SEQ differentially expressed genes ion transport cellular immunity
• 年，卷（期）: 2020,（3）
• 页码范围: 493-501
• 页数: 9页
• 分类号: R73

摘要

Transferrin receptor 1(TfR1),encoded by the TFRC gene,is the gatekeeper of cellular iron uptake for cells.A variety of molecular mechanisms are at work to tightly regulate TfR1 expression,and abnormal TfR1 expression has been associated with various diseases.In the current study,to determine the regulation pattern of TfR1,we cloned and overexpressed the human TFRC gene in HeLa cells.RNA-sequencing(RNA-seq)was used to analyze the global transcript levels in overexpressed(OE)and normal control(NC)samples.A total of 1669 differentially expressed genes(DEGs)were identified between OE and NC.Gene ontology(GO)analysis was carried out to explore the functions of the DEGs.It was found that multiple DEGs were associated with ion transport and immunity.Moreover,the regulatory network was constructed on basis of DEGs associated with ion transport and immunity,highlighting that TFRC was the node gene of the network.These results together suggested that precisely controlled TfR1 expression might be not only essential for iron homeostasis,but also globally important for cell physiology,including ion transport and immunity.