In this article,we derive the Lp-boundedness of the variation operators associated with the heat semigroup which is generated by the high order Schr(o)dinger type operator (-△)2 + V2 in]Rn(n ≥ 5) with V being a nonnegative potential satisfying the reverse H(o)lder inequality.Furthermore,we prove the boundedness of the variation operators on associated Morrey spaces.In the proof of the main results,we always make use of the variation inequalities associated with the heat semigroup generated by the biharmonic operator (-△)2.